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dapi stain solution  (Vector Laboratories)


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    Vector Laboratories dapi stain solution
    Dapi Stain Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mounting+medium+with+dapi+solution/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1253_slash_circj__cj___25___0709-33-12-16
    Average 98 stars, based on 21989 article reviews
    dapi stain solution - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke.
    Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X-100 for 1 h, and then sections were incubated overnight with antiIba1 (WaKo, Cat 019–19741, 1:200) antibody, anti-GFAP (CST, 3670, 1:300) antibody, anti-MAP2 (Abcam, ab32454, 1:200) antibody, anti-ARG1 (Abcam, ab239731, 1:200) antibody, anti-iNOS (CAYMAN, 160862, 1:200) antibody, anti-HIF-1 α (Abcam, ab8366, 1:200) antibody and anti-TIM-3 (ABclonal, A2516, 1:200) antibody at 4◦C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye-conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with DAPI staining solution (VECTOR LABORATORIES, H-1200). .. Images were obtained by inverted fluorescence microscopy, and ImageJ software was used to analyze the images.

    Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke
    Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X‐100 for 1 h, and then sections were incubated overnight with anti‐Iba1 (WaKo, Cat 019–19741, 1:200) antibody, anti‐GFAP (CST, 3670, 1:300) antibody, anti‐MAP2 (Abcam, ab32454, 1:200) antibody, anti‐ARG1 (Abcam, ab239731, 1:200) antibody, anti‐iNOS (CAYMAN, 160862, 1:200) antibody, anti‐HIF‐1α (Abcam, ab8366, 1:200) antibody and anti‐TIM‐3 (ABclonal, A2516, 1:200) antibody at 4°C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye‐conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with DAPI staining solution (VECTOR LABORATORIES, H‐1200). .. Images were obtained by inverted fluorescence microscopy, and ImageJ software was used to analyze the images.

    Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ―
    Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with DAPI stain solution (H-1200-10; Vector Laboratories, USA) and incubated for 5 min at room temperature in the dark to visualize nuclei. .. Two slides (6 sections each) per group were assessed under a Leica SP8 confocal microscope.

    Staining:

    Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke.
    Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X-100 for 1 h, and then sections were incubated overnight with antiIba1 (WaKo, Cat 019–19741, 1:200) antibody, anti-GFAP (CST, 3670, 1:300) antibody, anti-MAP2 (Abcam, ab32454, 1:200) antibody, anti-ARG1 (Abcam, ab239731, 1:200) antibody, anti-iNOS (CAYMAN, 160862, 1:200) antibody, anti-HIF-1 α (Abcam, ab8366, 1:200) antibody and anti-TIM-3 (ABclonal, A2516, 1:200) antibody at 4◦C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye-conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with DAPI staining solution (VECTOR LABORATORIES, H-1200). .. Images were obtained by inverted fluorescence microscopy, and ImageJ software was used to analyze the images.

    Article Title: Prolonged dysregulation and pathological changes in the upper respiratory tract of SARS-CoV-2 infected hamsters
    Article Snippet: .. FITC-conjugated (Invitrogen, 65-6111) secondary antibodies were used for immunofluorescent staining, while the nuclei were counterstained with VECTASHIELD Antifade Mounting Solution/DAPI (Vector Laboratories). .. TUNEL staining was performed for in situ apoptosis detection by using Click-iT Plus TUNEL Assay kit (Invitrogen, C10617 ).

    Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke
    Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X‐100 for 1 h, and then sections were incubated overnight with anti‐Iba1 (WaKo, Cat 019–19741, 1:200) antibody, anti‐GFAP (CST, 3670, 1:300) antibody, anti‐MAP2 (Abcam, ab32454, 1:200) antibody, anti‐ARG1 (Abcam, ab239731, 1:200) antibody, anti‐iNOS (CAYMAN, 160862, 1:200) antibody, anti‐HIF‐1α (Abcam, ab8366, 1:200) antibody and anti‐TIM‐3 (ABclonal, A2516, 1:200) antibody at 4°C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye‐conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with DAPI staining solution (VECTOR LABORATORIES, H‐1200). .. Images were obtained by inverted fluorescence microscopy, and ImageJ software was used to analyze the images.

    Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ―
    Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with DAPI stain solution (H-1200-10; Vector Laboratories, USA) and incubated for 5 min at room temperature in the dark to visualize nuclei. .. Two slides (6 sections each) per group were assessed under a Leica SP8 confocal microscope.

    Article Title: Post-stroke electroacupuncture promotes cerebrovascular repair in the peri-infarct cortex of ischemic stroke mice
    Article Snippet: .. Chemicals and reagents CD31 antibody (R&D Systems, Minneapolis, USA), Collagen V antibody (ab19808, Abcam, Cambridge, UK), NeuN antibody ab104224, Abcam, Cambridge, UK), Nissl stain (MP21480, Thermo isher, USA), Ki-67 antibody (PA5-19462, Invitrogen, Carlsbad, USA), lexa Fluor-conjugated secondary antibodies (Invitrogen, Carlsbad, SA), Tomato lectin (DL-1177, Vector Laboratories, Burlingame, SA), and DAPI staining solution (H1200, Vector Laboratories, urlingame, USA). ..

    other:

    Article Title: Prolonged dysregulation and pathological changes in the upper respiratory tract of SARS-CoV-2 infected hamsters.
    Article Snippet: Determination of viral load by real-time reverse-transcription polymerase chain reaction (RT-qPCR) and fifty percent tissue culture infectious doses (TCID50) assay Lung and nasal turbinate tissues were homogenized, and total RNA was extracted with MiniBEST Universal RNA Extraction Kit (9767A, Takara).

    DNA Extraction:

    Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease
    Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with DAPI Vectorlabs H-1200 BES-H2O2-Ac solution Wako 028-17811 1-phenyl-2-thiourea Sigma-Aldrich P7629 tricaine methanesulfonate, Sigma-Aldrich E10521 methylcellulose Sigma-Aldrich 217277 Matrigel-coated plates Corning 354234 mTeSR1 medium Stemcell 85851 Neurobasal medium Cellapy CA1001500 DME/F-12 Cytiva SH30023.01 nerve growth factor (N2) Stemcell 07152 B27 Gibco 17504-044 L-GLU Sigma-Aldrich G8540 2ME Gibco 21985-023 b-FGF Stemcell 78003 EGF Stemcell 78006.1 Accutase Sigma-Aldrich A6964 BSA FUDE biological FD0030-10 Matrigel-coated 12-well cell culture plate Corning Costar 3513 Scrather tool SPL LIFESCIENCE 201925 Matrigel-coated 8μm cell culture inserts Corning 353097 Crystal violet Tianjin Damao 548-62-9 Pageruler prestained protein ladder (10-180kDa) Thermo Scientific 26616 Prestained protein marker II (10-200 kDa) Servicebio G2058 3. ..

    Cell Culture:

    Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease
    Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with DAPI Vectorlabs H-1200 BES-H2O2-Ac solution Wako 028-17811 1-phenyl-2-thiourea Sigma-Aldrich P7629 tricaine methanesulfonate, Sigma-Aldrich E10521 methylcellulose Sigma-Aldrich 217277 Matrigel-coated plates Corning 354234 mTeSR1 medium Stemcell 85851 Neurobasal medium Cellapy CA1001500 DME/F-12 Cytiva SH30023.01 nerve growth factor (N2) Stemcell 07152 B27 Gibco 17504-044 L-GLU Sigma-Aldrich G8540 2ME Gibco 21985-023 b-FGF Stemcell 78003 EGF Stemcell 78006.1 Accutase Sigma-Aldrich A6964 BSA FUDE biological FD0030-10 Matrigel-coated 12-well cell culture plate Corning Costar 3513 Scrather tool SPL LIFESCIENCE 201925 Matrigel-coated 8μm cell culture inserts Corning 353097 Crystal violet Tianjin Damao 548-62-9 Pageruler prestained protein ladder (10-180kDa) Thermo Scientific 26616 Prestained protein marker II (10-200 kDa) Servicebio G2058 3. ..

    Marker:

    Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease
    Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with DAPI Vectorlabs H-1200 BES-H2O2-Ac solution Wako 028-17811 1-phenyl-2-thiourea Sigma-Aldrich P7629 tricaine methanesulfonate, Sigma-Aldrich E10521 methylcellulose Sigma-Aldrich 217277 Matrigel-coated plates Corning 354234 mTeSR1 medium Stemcell 85851 Neurobasal medium Cellapy CA1001500 DME/F-12 Cytiva SH30023.01 nerve growth factor (N2) Stemcell 07152 B27 Gibco 17504-044 L-GLU Sigma-Aldrich G8540 2ME Gibco 21985-023 b-FGF Stemcell 78003 EGF Stemcell 78006.1 Accutase Sigma-Aldrich A6964 BSA FUDE biological FD0030-10 Matrigel-coated 12-well cell culture plate Corning Costar 3513 Scrather tool SPL LIFESCIENCE 201925 Matrigel-coated 8μm cell culture inserts Corning 353097 Crystal violet Tianjin Damao 548-62-9 Pageruler prestained protein ladder (10-180kDa) Thermo Scientific 26616 Prestained protein marker II (10-200 kDa) Servicebio G2058 3. ..



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