dapi stain solution (Vector Laboratories)
98
Structured Review
Vector Laboratories
dapi stain solution
Dapi Stain Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mounting+medium+with+dapi+solution/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1253_slash_circj__cj___25___0709-33-12-16
Average 98 stars, based on 21989 article reviews
Dapi Stain Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21989 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mounting+medium+with+dapi+solution/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1253_slash_circj__cj___25___0709-33-12-16
Average 98 stars, based on 21989 article reviews
dapi stain solution - by Bioz Stars,
2026-10
98/100 stars
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Incubation:Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke. Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X-100 for 1 h, and then sections were incubated overnight with antiIba1 (WaKo, Cat 019–19741, 1:200) antibody, anti-GFAP (CST, 3670, 1:300) antibody, anti-MAP2 (Abcam, ab32454, 1:200) antibody, anti-ARG1 (Abcam, ab239731, 1:200) antibody, anti-iNOS (CAYMAN, 160862, 1:200) antibody, anti-HIF-1 α (Abcam, ab8366, 1:200) antibody and anti-TIM-3 (ABclonal, A2516, 1:200) antibody at 4◦C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye-conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X‐100 for 1 h, and then sections were incubated overnight with anti‐Iba1 (WaKo, Cat 019–19741, 1:200) antibody, anti‐GFAP (CST, 3670, 1:300) antibody, anti‐MAP2 (Abcam, ab32454, 1:200) antibody, anti‐ARG1 (Abcam, ab239731, 1:200) antibody, anti‐iNOS (CAYMAN, 160862, 1:200) antibody, anti‐HIF‐1α (Abcam, ab8366, 1:200) antibody and anti‐TIM‐3 (ABclonal, A2516, 1:200) antibody at 4°C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye‐conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ― Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with Staining:Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke. Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X-100 for 1 h, and then sections were incubated overnight with antiIba1 (WaKo, Cat 019–19741, 1:200) antibody, anti-GFAP (CST, 3670, 1:300) antibody, anti-MAP2 (Abcam, ab32454, 1:200) antibody, anti-ARG1 (Abcam, ab239731, 1:200) antibody, anti-iNOS (CAYMAN, 160862, 1:200) antibody, anti-HIF-1 α (Abcam, ab8366, 1:200) antibody and anti-TIM-3 (ABclonal, A2516, 1:200) antibody at 4◦C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye-conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with Article Title: Prolonged dysregulation and pathological changes in the upper respiratory tract of SARS-CoV-2 infected hamsters Article Snippet: .. FITC-conjugated (Invitrogen, 65-6111) secondary antibodies were used for immunofluorescent staining, while the nuclei were counterstained with Article Title: Iron Oxide Nanozyme as Reactive Oxygen and Nitrogen Species Scavenger to Regulate Microglial Homeostasis in Stroke Article Snippet: Sections were incubated with goat serum containing 0.4% Triton X‐100 for 1 h, and then sections were incubated overnight with anti‐Iba1 (WaKo, Cat 019–19741, 1:200) antibody, anti‐GFAP (CST, 3670, 1:300) antibody, anti‐MAP2 (Abcam, ab32454, 1:200) antibody, anti‐ARG1 (Abcam, ab239731, 1:200) antibody, anti‐iNOS (CAYMAN, 160862, 1:200) antibody, anti‐HIF‐1α (Abcam, ab8366, 1:200) antibody and anti‐TIM‐3 (ABclonal, A2516, 1:200) antibody at 4°C. .. After washing with TBST, sections were incubated with FITC and TRITC fluorescent dye‐conjugated secondary antibody (1:200, Jackson Laboratories) for 1 h. Finally, the nuclei were stained with Article Title: Targeting Nuclear Factor-κB1 With Andrographolide ― Novel Strategy to Inhibit Vascular Lesions and Aneurysm Formation in Abdominal Aortic Aneurysm ― Article Snippet: After the overnight incubation, samples were washed three times with PBS (5 min each) and then incubated with secondary antibodies (Alexa Fluor 488/594/647-conjugated IgG secondary antibodies; 1 : 200 dilution; A-11008/A-11001/A-11012/A-11032; Invitrogen) for 60 min at room temperature. .. After washing with PBS, samples were covered with Antifade Mounting Medium with Article Title: Post-stroke electroacupuncture promotes cerebrovascular repair in the peri-infarct cortex of ischemic stroke mice Article Snippet: .. Chemicals and reagents CD31 antibody (R&D Systems, Minneapolis, USA), Collagen V antibody (ab19808, Abcam, Cambridge, UK), NeuN antibody ab104224, Abcam, Cambridge, UK), Nissl stain (MP21480, Thermo isher, USA), Ki-67 antibody (PA5-19462, Invitrogen, Carlsbad, USA), lexa Fluor-conjugated secondary antibodies (Invitrogen, Carlsbad, SA), Tomato lectin (DL-1177, Vector Laboratories, Burlingame, SA), and other:Article Title: Prolonged dysregulation and pathological changes in the upper respiratory tract of SARS-CoV-2 infected hamsters. Article Snippet: Determination of viral load by real-time reverse-transcription polymerase chain reaction (RT-qPCR) and fifty percent tissue culture infectious doses (TCID50) assay Lung and nasal turbinate tissues were homogenized, and total RNA was extracted with MiniBEST Universal RNA Extraction Kit (9767A, Takara). DNA Extraction:Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with Cell Culture:Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with Marker:Article Title: Integrated genomic analysis of EDNRB common and rare variants in Hirschsprung disease Article Snippet: Antibodies HuC/HuD Monoclonal Antibody (16A11) ThermoFisher A21271 Alexa Fluor 568 goat anti mouse IgG Abcam ab175473 Anti-GAPDH antibody [6C5] – Loading Control Abcam ab8245 Goat Anti-Mouse IgG H&L (HRP) Abcam ab205719 Anti-Endothelin B Receptor/ET-B antibody Abcam ab129102 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam ab150077 Goat Anti-Rabbit IgG H&L (HRP) Abcam ab205718 2. .. Other reagents and consumables Blood Genomic DNA Extraction Kit TIANGEN DP348-03 2 × Rapid Taq Master Mix Vazyme P222-02 TritonX-100 Sigma-Aldrich T8787 DMSO MPBIO 196055 VECTASHIELD with |